Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 06/08/2016 | Bradford | 0 | 0 | Port Vale | D Deadman |
| 13/08/2016 | Port Vale | 2 | 0 | Southend | L Probert |
| 16/08/2016 | Port Vale | 1 | 0 | Rochdale | R Jones |
| 20/08/2016 | Swindon | 1 | 0 | Port Vale | D Whitestone |
| 27/08/2016 | Port Vale | 3 | 1 | Scunthorpe | B Malone |
| 03/09/2016 | Bury | 4 | 1 | Port Vale | R Joyce |
| 10/09/2016 | Peterboro | 2 | 2 | Port Vale | G Horwood |
| 17/09/2016 | Port Vale | 2 | 1 | Gillingham | C Boyeson |
| 24/09/2016 | Bristol Rvs | 2 | 1 | Port Vale | T Kettle |
| 27/09/2016 | Port Vale | 3 | 1 | Millwall | J Simpson |
| 01/10/2016 | Port Vale | 0 | 2 | Coventry | J Busby |
| 09/10/2016 | Milton Keynes Dons | 0 | 1 | Port Vale | S Stockbridge |
| 15/10/2016 | Sheffield United | 4 | 0 | Port Vale | M Heywood |
| 18/10/2016 | Port Vale | 1 | 1 | Charlton | G Salisbury |
| 22/10/2016 | Port Vale | 2 | 2 | Oxford | B Toner |
| 29/10/2016 | Bolton | 3 | 1 | Port Vale | N Miller |
| 12/11/2016 | Port Vale | 2 | 1 | Fleetwood Town | J Brooks |
| 19/11/2016 | Charlton | 2 | 0 | Port Vale | R Clark |
| 22/11/2016 | Port Vale | 2 | 2 | Oldham | D England |
| 26/11/2016 | Shrewsbury | 0 | 0 | Port Vale | S Duncan |
| 10/12/2016 | Port Vale | 2 | 3 | Northampton | G Salisbury |
| 17/12/2016 | AFC Wimbledon | 4 | 0 | Port Vale | B Malone |
| 26/12/2016 | Port Vale | 0 | 1 | Walsall | D Deadman |
| 30/12/2016 | Port Vale | 1 | 0 | Chesterfield | M Salisbury |
| 02/01/2017 | Oldham | 0 | 0 | Port Vale | R Clark |
| 20/01/2017 | Port Vale | 2 | 2 | Bury | O Langford |
| 28/01/2017 | Scunthorpe | 3 | 2 | Port Vale | D England |
| 04/02/2017 | Port Vale | 0 | 3 | Peterboro | C Breakspear |
| 11/02/2017 | Gillingham | 1 | 1 | Port Vale | N Kinseley |
| 14/02/2017 | Millwall | 2 | 0 | Port Vale | D Drysdale |
| 18/02/2017 | Port Vale | 1 | 1 | Bristol Rvs | D Whitestone |
| 25/02/2017 | Port Vale | 1 | 2 | Bradford | M Heywood |
| 04/03/2017 | Southend | 1 | 1 | Port Vale | N Miller |
| 11/03/2017 | Port Vale | 3 | 2 | Swindon | G Eltringham |
| 14/03/2017 | Northampton | 2 | 1 | Port Vale | G Ward |
| 17/03/2017 | Port Vale | 2 | 1 | Shrewsbury | R Joyce |
| 21/03/2017 | Coventry | 2 | 1 | Port Vale | D Webb |
| 25/03/2017 | Port Vale | 0 | 0 | Milton Keynes Dons | K Johnson |
| 01/04/2017 | Port Vale | 2 | 0 | AFC Wimbledon | D Drysdale |
| 04/04/2017 | Rochdale | 3 | 0 | Port Vale | S Stockbridge |
| 08/04/2017 | Chesterfield | 1 | 0 | Port Vale | R Clark |
| 14/04/2017 | Port Vale | 0 | 3 | Sheffield United | N Kinseley |
| 17/04/2017 | Oxford | 2 | 0 | Port Vale | T Kettle |
| 22/04/2017 | Port Vale | 0 | 2 | Bolton | C Boyeson |
| 25/04/2017 | Walsall | 0 | 1 | Port Vale | J Busby |
| 30/04/2017 | Fleetwood Town | 0 | 0 | Port Vale | R Joyce |